Urolithiasis, commonly known as kidney stones, is a global health problem. This study investigated the in vitro anti-urolithiatic activity of Delonix regia ethanolic extract (EEDR) compared to F1 and F2. established formulations for urolithiasis. Nucleation and aggregation assays were conducted to assess the effects on calcium oxalate (CaOx) crystal formation. EEDR exhibited dose-dependent inhibition of both nucleation and aggregation, showing highest inhibition at 1000 µg/ml. Polyherbal formulation F1 displayed sustained inhibition throughout the observed time points, F2 showed a significant effect primarily within the first hour. These findings suggest that Delonix regia has potential as a therapeutic intervention for urolithiasis. Further research, including in vivo studies, is warranted to explore its clinical applications.

Mrs. Mamta V. Venna, Mr. Pratheesh A. Arul, Ms. Kishori Parab, Ms. Shrutika Pillai, Ms. Dhanashree Kakade

Delonix regia, nucleation assay, aggregation assay, calcium oxalate, kidney stone